Part:BBa_K5403004

Outer membrane protein A (OmpA)

The outer membrane protein A (OmpA) is found in E. coli and has been used as an anchor to attach other proteins to the membrane exterior by recombinant expression (Beck et al., 2017). A protein of interest can be attached to the C-terminus of OmpA, with a flexible linker in between. Composite part BBa_5403013 was made based on this basic part, with the aim of displaying GFP on the membrane, anchored onto OmpA.

References

Beck, B. R., Lee, S. H., Kim, D., Park, J. H., Lee, H. K., Kwon, S., Lee, K. H., Lee, J. I., & Song, S. K. (2017). A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines. Fish & Shellfish Immunology, 68, 19–28. https://doi.org/10.1016/j.fsi.2017.07.004

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 412
  Illegal AgeI site found at 566
  Illegal AgeI site found at 591
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 528
  Illegal SapI.rc site found at 827